Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

All Photons Imaging Through Volumetric Scattering.

ABSTRACT: Imaging through thick highly scattering media (sample thickness ? mean free path) can realize broad applications in biomedical and industrial imaging as well as remote sensing. Here we propose a computational "All Photons Imaging" (API) framework that utilizes time-resolved measurement for imaging through thick volumetric scattering by using both early arrived (non-scattered) and diffused photons. As opposed to other methods which aim to lock on specific photons (coherent, ballistic, acoustically modulated, etc.), this framework aims to use all of the optical signal. Compared to conventional early photon measurements for imaging through a 15?mm tissue phantom, our method shows a two fold improvement in spatial resolution (4db increase in Peak SNR). This all optical, calibration-free framework enables widefield imaging through thick turbid media, and opens new avenues in non-invasive testing, analysis, and diagnosis.

SUBMITTER: Satat G

PROVIDER: S-EPMC5041145 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Publications

All Photons Imaging Through Volumetric Scattering.

Satat Guy G Heshmat Barmak B Raviv Dan D Raskar Ramesh R

Scientific reports 20160929

Imaging through thick highly scattering media (sample thickness ≫ mean free path) can realize broad applications in biomedical and industrial imaging as well as remote sensing. Here we propose a computational "All Photons Imaging" (API) framework that utilizes time-resolved measurement for imaging through thick volumetric scattering by using both early arrived (non-scattered) and diffused photons. As opposed to other methods which aim to lock on specific photons (coherent, ballistic, acousticall ...[more]

PMID: 27683065

Similar Datasets

Volumetric chemical imaging by clearing-enhanced stimulated Raman scattering microscopy.

Project description:Three-dimensional visualization of tissue structures using optical microscopy facilitates the understanding of biological functions. However, optical microscopy is limited in tissue penetration due to severe light scattering. Recently, a series of tissue-clearing techniques have emerged to allow significant depth-extension for fluorescence imaging. Inspired by these advances, we develop a volumetric chemical imaging technique that couples Raman-tailored tissue-clearing with stimulated Raman scattering (SRS) microscopy. Compared with the standard SRS, the clearing-enhanced SRS achieves greater than 10-times depth increase. Based on the extracted spatial distribution of proteins and lipids, our method reveals intricate 3D organizations of tumor spheroids, mouse brain tissues, and tumor xenografts. We further develop volumetric phasor analysis of multispectral SRS images for chemically specific clustering and segmentation in 3D. Moreover, going beyond the conventional label-free paradigm, we demonstrate metabolic volumetric chemical imaging, which allows us to simultaneously map out metabolic activities of protein and lipid synthesis in glioblastoma. Together, these results support volumetric chemical imaging as a valuable tool for elucidating comprehensive 3D structures, compositions, and functions in diverse biological contexts, complementing the prevailing volumetric fluorescence microscopy.

| S-EPMC6452712 | biostudies-literature

Exploiting volumetric wave correlation for enhanced depth imaging in scattering medium.

Project description:Imaging an object embedded within a scattering medium requires the correction of complex sample-induced wave distortions. Existing approaches have been designed to resolve them by optimizing signal waves recorded in each 2D image. Here, we present a volumetric image reconstruction framework that merges two fundamental degrees of freedom, the wavelength and propagation angles of light waves, based on the object momentum conservation principle. On this basis, we propose methods for exploiting the correlation of signal waves from volumetric images to better cope with multiple scattering. By constructing experimental systems scanning both wavelength and illumination angle of the light source, we demonstrated a 32-fold increase in the use of signal waves compared with that of existing 2D-based approaches and achieved ultrahigh volumetric resolution (lateral resolution: 0.41 [Formula: see text], axial resolution: 0.60 [Formula: see text]) even within complex scattering medium owing to the optimal coherent use of the broad spectral bandwidth (225 nm).

| S-EPMC10073116 | biostudies-literature

Adaptive volumetric light and atmospheric scattering.

Project description:An adaptive sampling-based atmospheric scattering and volumetric light framework for flight simulator (FS) is proposed to enhance the immersion and realism in real-time. The framework comprises epipolar sampling (ES), visible factor culling (VFC), interactive participating media density estimating (IPMDE). The main process of proposed architecture is as follows: the scene is divided into two levels according to the distance from the camera. In the high-level pipeline, the layer close to the camera, more samples, and smaller sampling step size is used to improve image quality. Further, the IPMDE method is designed to enhance realism by achieving interactivity with the participating media and multiple light sources. Further optimization is performed by the lookup table and 3D volumetric textures, by which we can compute the density of participating media and the scattering coefficient in parallel. In the low-level pipeline, when the samples are far away from the camera, its influence on the final output is also reduced, which results in fewer samples and a bigger sampling step size. The improved ES method further reduces the number of samples involved in ray marching using the features of epipolar geometry. It then generates global light effects and shadows of distant terrain. The VFC method uses an acceleration structure to quickly find the lit segments which eliminate samples blocked by obstacles. The experimental results demonstrate our architecture achieves a better sense of reality in real-time and is very suitable for FS.

| S-EPMC7673549 | biostudies-literature

Volumetric imaging and quantification of cytoarchitecture and myeloarchitecture with intrinsic scattering contrast.

Project description:We present volumetric imaging and computational techniques to quantify neuronal and myelin architecture with intrinsic scattering contrast. Using spectral / Fourier domain Optical Coherence Microscopy (OCM) and software focus-tracking we validate imaging of neuronal cytoarchitecture and demonstrate quantification in the rodent cortex in vivo. Additionally, by ex vivo imaging in conjunction with optical clearing techniques, we demonstrate that intrinsic scattering contrast is preserved in the brain, even after sacrifice and fixation. We volumetrically image cytoarchitecture and myeloarchitecture ex vivo across the entire depth of the rodent cortex. Cellular-level imaging up to the working distance of our objective (~3 mm) is demonstrated ex vivo. Architectonic features show the expected laminar characteristics; moreover, changes in contrast after the application of acetic acid suggest that entire neuronal cell bodies are responsible for the "negative contrast" present in the images. Clearing and imaging techniques that preserve tissue architectural integrity have the potential to enable non-invasive studies of the brain during development, disease, and remodeling, even in samples where exogenous labeling is impractical.

| S-EPMC3799660 | biostudies-other

Probing the spectral density of the surface electromagnetic fields through scattering of waveguide photons.

Project description:The spectral density of the metal-surface electromagnetic fields will be strongly modified in the presence of a closely-spaced quantum emitter. In this work, we propose a feasible way to probe the changes of the spectral density through the scattering of the waveguide photon incident on the quantum emitter. The variances of the lineshape in the transmission spectra indicate the coherent interaction between the emitter and the pseudomode resulting from all the surface electromagnetic modes. We further investigate the quantum coherence between the emitter and the pseudomode of the metal-dielectric interface.

| S-EPMC4748287 | biostudies-other

Wide-field multiphoton imaging through scattering media without correction.

Project description:Optical approaches to fluorescent, spectroscopic, and morphological imaging have made exceptional advances in the last decade. Super-resolution imaging and wide-field multiphoton imaging are now underpinning major advances across the biomedical sciences. While the advances have been startling, the key unmet challenge to date in all forms of optical imaging is to penetrate deeper. A number of schemes implement aberration correction or the use of complex photonics to address this need. In contrast, we approach this challenge by implementing a scheme that requires no a priori information about the medium nor its properties. Exploiting temporal focusing and single-pixel detection in our innovative scheme, we obtain wide-field two-photon images through various turbid media including a scattering phantom and tissue reaching a depth of up to seven scattering mean free path lengths. Our results show that it competes favorably with standard point-scanning two-photon imaging, with up to a fivefold improvement in signal-to-background ratio while showing significantly lower photobleaching.

| S-EPMC6184782 | biostudies-literature

Non-invasive super-resolution imaging through dynamic scattering media.

Project description:Super-resolution imaging has been revolutionizing technical analysis in various fields from biological to physical sciences. However, many objects are hidden by strongly scattering media such as biological tissues that scramble light paths, create speckle patterns and hinder object's visualization, let alone super-resolution imaging. Here, we demonstrate non-invasive super-resolution imaging through scattering media based on a stochastic optical scattering localization imaging (SOSLI) technique. After capturing multiple speckle patterns of photo-switchable point sources, our computational approach utilizes the speckle correlation property of scattering media to retrieve an image with a 100-nm resolution, an eight-fold enhancement compared to the diffraction limit. More importantly, we demonstrate our SOSLI to do non-invasive super-resolution imaging through not only static scattering media, but also dynamic scattering media with strong decorrelation such as biological tissues. Our approach paves the way to non-invasively visualize various samples behind scattering media at nanometer levels of detail.

| S-EPMC8149393 | biostudies-literature

Real-time imaging through strongly scattering media: seeing through turbid media, instantly.

Project description:Numerous everyday situations like navigation, medical imaging and rescue operations require viewing through optically inhomogeneous media. This is a challenging task as photons propagate predominantly diffusively (rather than ballistically) due to random multiple scattering off the inhomogenieties. Real-time imaging with ballistic light under continuous-wave illumination is even more challenging due to the extremely weak signal, necessitating voluminous data-processing. Here we report imaging through strongly scattering media in real-time and at rates several times the critical flicker frequency of the eye, so that motion is perceived as continuous. Two factors contributed to the speedup of more than three orders of magnitude over conventional techniques - the use of a simplified algorithm enabling processing of data on the fly, and the utilisation of task and data parallelization capabilities of typical desktop computers. The extreme simplicity of the technique, and its implementation with present day low-cost technology promises its utility in a variety of devices in maritime, aerospace, rail and road transport, in medical imaging and defence. It is of equal interest to the common man and adventure sportsperson like hikers, divers, mountaineers, who frequently encounter situations requiring realtime imaging through obscuring media. As a specific example, navigation under poor visibility is examined.

| S-EPMC4844949 | biostudies-literature

Structure determination from single molecule X-ray scattering with three photons per image.

Project description:Scattering experiments with femtosecond high-intensity free-electron laser pulses provide a new route to macromolecular structure determination. While currently limited to nano-crystals or virus particles, the ultimate goal is scattering on single biomolecules. The main challenges in these experiments are the extremely low signal-to-noise ratio due to the very low expected photon count per scattering image, often well below 100, as well as the random orientation of the molecule in each shot. Here we present a de novo correlation-based approach and show that three coherently scattered photons per image suffice for structure determination. Using synthetic scattering data of a small protein, we demonstrate near-atomic resolution of 3.3 Å using 3.3 × 1010 coherently scattered photons from 3.3 × 109 images, which is within experimental reach. Further, our three-photon correlation approach is robust to additional noise from incoherent scattering; the number of disordered solvent molecules attached to the macromolecular surface should be kept small.

| S-EPMC6006178 | biostudies-literature

Controlling Light in Scattering Materials for Volumetric Additive Manufacturing.

Project description:3D printing has revolutionized the manufacturing of volumetric components and structures in many areas. Several fully volumetric light-based techniques have been recently developed thanks to the advent of photocurable resins, promising to reach unprecedented short print time (down to a few tens of seconds) while keeping a good resolution (around 100 μm). However, these new approaches only work with homogeneous and relatively transparent resins so that the light patterns used for photo-polymerization are not scrambled along their propagation. Herein, a method that takes into account light scattering in the resin prior to computing projection patterns is proposed. Using a tomographic volumetric printer, it is experimentally demonstrated that implementation of this correction is critical when printing objects whose size exceeds the scattering mean free path. To show the broad applicability of the technique, functional objects of high print fidelity are fabricated in hard organic scattering acrylates and soft cell-laden hydrogels (at 4 million cells mL-1 ). This opens up promising perspectives in printing inside turbid materials with particular interesting applications for bioprinting cell-laden constructs.

| S-EPMC9353445 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data