Ontology highlight
ABSTRACT: Background
Chikungunya virus (CHIKV) is a mosquito-borne virus currently transmitted in about 60 countries. CHIKV causes acute flu-like symptoms and in many cases prolonged musculoskeletal and joint pain. Detection of the infection is mostly done using RT-RCR or ELISA, which are not suitable for point-of-care diagnosis.Methodology/principal findings
In this study, a reverse transcription recombinase polymerase amplification (RT-RPA) assay for the detection of the CHIKV was developed. The assay sensitivity, specificity, and cross-reactivity were tested. CHIKV RT-RPA assay detected down to 80 genome copies/reaction in a maximum of 15 minutes. It successfully identified 18 isolates representing the three CHIKV genotypes. No cross-reactivity was detected to other alphaviruses and arboviruses except O'nyong'nyong virus, which could be differentiated by a modified RPA primer pair. Seventy-eight samples were screened both by RT-RPA and real-time RT-PCR. The diagnostic sensitivity and specificity of the CHIKV RT-RPA assay were determined at 100%.Conclusions/significance
The developed RT-RPA assay represents a promising method for the molecular detection of CHIKV at point of need.
SUBMITTER: Patel P
PROVIDER: S-EPMC5042537 | biostudies-literature | 2016 Sep
REPOSITORIES: biostudies-literature
PLoS neglected tropical diseases 20160929 9
<h4>Background</h4>Chikungunya virus (CHIKV) is a mosquito-borne virus currently transmitted in about 60 countries. CHIKV causes acute flu-like symptoms and in many cases prolonged musculoskeletal and joint pain. Detection of the infection is mostly done using RT-RCR or ELISA, which are not suitable for point-of-care diagnosis.<h4>Methodology/principal findings</h4>In this study, a reverse transcription recombinase polymerase amplification (RT-RPA) assay for the detection of the CHIKV was develo ...[more]