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XA21-specific induction of stress-related genes following Xanthomonas infection of detached rice leaves.

ABSTRACT: The rice XA21 receptor kinase confers robust resistance to the bacterial pathogen Xanthomonas oryzaepv. oryzae (Xoo). We developed a detached leaf infection assay to quickly and reliably measure activation of the XA21-mediated immune response using genetic markers. We used RNA sequencing of elf18 treated EFR:XA21:GFP plants to identify candidate genes that could serve as markers for XA21 activation. From this analysis, we identified eight genes that are up-regulated in both in elf18 treated EFR:XA21:GFP rice leaves and Xoo infected XA21 rice leaves. These results provide a rapid and reliable method to assess bacterial-rice interactions.

SUBMITTER: Thomas NC

PROVIDER: S-EPMC5045893 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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XA21-specific induction of stress-related genes following Xanthomonas infection of detached rice leaves.

Thomas Nicholas C NC Schwessinger Benjamin B Liu Furong F Chen Huamin H Wei Tong T Nguyen Yen P YP Shaker Isaac W F IWF Ronald Pamela C PC

PeerJ 20160928

The rice XA21 receptor kinase confers robust resistance to the bacterial pathogen Xanthomonas oryzaepv. oryzae (Xoo). We developed a detached leaf infection assay to quickly and reliably measure activation of the XA21-mediated immune response using genetic markers. We used RNA sequencing of elf18 treated EFR:XA21:GFP plants to identify candidate genes that could serve as markers for XA21 activation. From this analysis, we identified eight genes that are up-regulated in both ...[more]

PMID: 27703843

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