Visualizing and mapping the cerebellum with serial optical coherence scanner.

ABSTRACT: We present the visualization of the mouse cerebellum and adjacent brainstem using a serial optical coherence scanner, which integrates a vibratome slicer and polarization-sensitive optical coherence tomography for ex vivo imaging. The scanner provides intrinsic optical contrasts to distinguish the cerebellar cortical layers and white matter. Images from serial scans reveal the large-scale anatomy in detail and map the nerve fiber pathways in the cerebellum and brainstem. By incorporating a water-immersion microscope objective, we also present high-resolution tiled images that delineate fine structures in the cerebellum and brainstem.

SUBMITTER: Liu CJ 

PROVIDER: S-EPMC5048104 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature