Ontology highlight
ABSTRACT:
SUBMITTER: Driggers CM
PROVIDER: S-EPMC5048534 | biostudies-literature | 2016 Oct
REPOSITORIES: biostudies-literature
Driggers Camden M CM Kean Kelsey M KM Hirschberger Lawrence L LL Cooley Richard B RB Stipanuk Martha H MH Karplus P Andrew PA
Journal of molecular biology 20160729 20
In mammals, the non-heme iron enzyme cysteine dioxygenase (CDO) helps regulate Cys levels through converting Cys to Cys sulfinic acid. Its activity is in part modulated by the formation of a Cys93-Tyr157 crosslink that increases its catalytic efficiency over 10-fold. Here, 21 high-resolution mammalian CDO structures are used to gain insight into how the Cys-Tyr crosslink promotes activity and how select competitive inhibitors bind. Crystal structures of crosslink-deficient C93A and Y157F variant ...[more]