Ontology highlight
ABSTRACT:
SUBMITTER: Wen K
PROVIDER: S-EPMC5052038 | biostudies-literature | 2016 Sep
REPOSITORIES: biostudies-literature
Wen Kejia K Yang Lijuan L Xiong Tuanlin T Di Chao C Ma Danhui D Wu Menghua M Xue Zhaoyu Z Zhang Xuedi X Long Li L Zhang Weimin W Zhang Jiaying J Bi Xiaolin X Dai Junbiao J Zhang Qiangfeng Q Lu Zhi John ZJ Gao Guanjun G
Genome research 20160811 9
Long noncoding RNAs (lncRNAs), a recently discovered class of cellular RNAs, play important roles in the regulation of many cellular developmental processes. Although lncRNAs have been systematically identified in various systems, most of them have not been functionally characterized in vivo in animal models. In this study, we identified 128 testis-specific Drosophila lncRNAs and knocked out 105 of them using an optimized three-component CRISPR/Cas9 system. Among the lncRNA knockouts, 33 (31%) e ...[more]