Binding of Vinculin to Lipid Membranes in Its Inhibited and Activated States.

ABSTRACT: Phosphoinositols are an important class of phospholipids that are involved in a myriad of cellular processes, from cell signaling to motility and adhesion. Vinculin (Vn) is a major adaptor protein that regulates focal adhesions in conjunction with PIP₂ in lipid membranes and other cytoskeletal components. The binding and unbinding transitions of Vn at the membrane interface are an important link to understanding the coordination of cell signaling and motility. Using different biophysical tools, including atomic force microscopy combined with confocal fluorescence microscopy and Fourier transform infrared spectroscopy, we studied the nanoscopic interactions of activated and autoinhibited states of Vn with lipid membranes. We hypothesize that a weak interaction occurs between Vn a

SUBMITTER: Dwivedi M 

PROVIDER: S-EPMC5052449 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature