Ontology highlight
ABSTRACT:
SUBMITTER: Gaitan-Penas H
PROVIDER: S-EPMC5052465 | biostudies-literature | 2016 Oct
REPOSITORIES: biostudies-literature
Biophysical journal 20161001 7
Volume-regulated anion channels (VRACs) play an important role in controlling cell volume by opening upon cell swelling. Recent work has shown that heteromers of LRRC8A with other LRRC8 members (B, C, D, and E) form the VRAC. Here, we used Xenopus oocytes as a simple system to study LRRC8 proteins. We discovered that adding fluorescent proteins to the C-terminus resulted in constitutive anion channel activity. Using these constructs, we reproduced previous findings indicating that LRRC8 heterome ...[more]