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Activation of Xer-recombination at dif: structural basis of the FtsK?-XerD interaction.


ABSTRACT: Bacterial chromosomes are most often circular DNA molecules. This can produce a topological problem; a genetic crossover from homologous recombination results in dimerization of the chromosome. A chromosome dimer is lethal unless resolved. A site-specific recombination system catalyses this dimer-resolution reaction at the chromosomal site dif. In Escherichia coli, two tyrosine-family recombinases, XerC and XerD, bind to dif and carry out two pairs of sequential strand exchange reactions. However, what makes the reaction unique among site-specific recombination reactions is that the first step, XerD-mediated strand exchange, relies on interaction with the very C-terminus of the FtsK DNA translocase. FtsK is a powerful molecular motor that functions in cell division, co-ordinating division with clearing chromosomal DNA from the site of septation and also acts to position the dif sites for recombination. This is a model system for unlinking, separating and segregating large DNA molecules. Here we describe the molecular detail of the interaction between XerD and FtsK that leads to activation of recombination as deduced from a co-crystal structure, biochemical and in vivo experiments. FtsK? interacts with the C-terminal domain of XerD, above a cleft where XerC is thought to bind. We present a model for activation of recombination based on structural data.

SUBMITTER: Keller AN 

PROVIDER: S-EPMC5052618 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature

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Activation of Xer-recombination at dif: structural basis of the FtsKγ-XerD interaction.

Keller Andrew N AN   Xin Yue Y   Boer Stephanie S   Reinhardt Jonathan J   Baker Rachel R   Arciszewska Lidia K LK   Lewis Peter J PJ   Sherratt David J DJ   Löwe Jan J   Grainge Ian I  

Scientific reports 20161006


Bacterial chromosomes are most often circular DNA molecules. This can produce a topological problem; a genetic crossover from homologous recombination results in dimerization of the chromosome. A chromosome dimer is lethal unless resolved. A site-specific recombination system catalyses this dimer-resolution reaction at the chromosomal site dif. In Escherichia coli, two tyrosine-family recombinases, XerC and XerD, bind to dif and carry out two pairs of sequential strand exchange reactions. Howeve  ...[more]

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