A capillary-based multiplexed isothermal nucleic acid-based test for sexually transmitted diseases in patients.
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ABSTRACT:
SUBMITTER: Xu G
PROVIDER: S-EPMC5058349 | biostudies-literature | 2016 Oct
REPOSITORIES: biostudies-literature
Publications
A capillary-based multiplexed isothermal nucleic acid-based test for sexually transmitted diseases in patients.
Xu Gaolian G Zhao Hang H Cooper Jonathan M JM Reboud Julien J
Chemical communications (Cambridge, England) 20161001 82
We demonstrate a multiplexed loop mediated isothermal amplification (LAMP) assay for infectious disease diagnostics, where the analytical process flow of target pathogens genomic DNA is performed manually by moving magnetic beads through a series of plugs in a capillary. Heat is provided by a water bath and the results are read by the naked eye, enabling applications in low resource settings. ...[more]
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