Ontology highlight
ABSTRACT:
SUBMITTER: Manifava M
PROVIDER: S-EPMC5059141 | biostudies-literature | 2016 Oct
REPOSITORIES: biostudies-literature
Manifava Maria M Smith Matthew M Rotondo Sergio S Walker Simon S Niewczas Izabella I Zoncu Roberto R Clark Jonathan J Ktistakis Nicholas T NT
eLife 20161011
Amino acids are essential activators of mTORC1 via a complex containing RAG GTPases, RAGULATOR and the vacuolar ATPase. Sensing of amino acids causes translocation of mTORC1 to lysosomes, an obligate step for activation. To examine the spatial and temporal dynamics of this translocation, we used live imaging of the mTORC1 component RAPTOR and a cell permeant fluorescent analogue of di-leucine methyl ester. Translocation to lysosomes is a transient event, occurring within 2 min of aa addition and ...[more]