Ontology highlight
ABSTRACT:
SUBMITTER: Wang D
PROVIDER: S-EPMC5059719 | biostudies-literature | 2016 Oct
REPOSITORIES: biostudies-literature
Wang Delong D Miyazono Ken-Ichi KI Tanokura Masaru M
Scientific reports 20161012
R.PabI is a type II restriction enzyme that recognizes the 5'-GTAC-3' sequence and belongs to the HALFPIPE superfamily. Although most restriction enzymes cleave phosphodiester bonds at specific sites by hydrolysis, R.PabI flips the guanine and adenine bases of the recognition sequence out of the DNA helix and hydrolyzes the N-glycosidic bond of the flipped adenine in a similar manner to DNA glycosylases. In this study, we determined the structure of R.PabI in complex with double-stranded DNA wit ...[more]