Dataset Information

Comparison of the bacterial communities in feces from wild versus housed sables (Martes zibellina) by high-throughput sequence analysis of the bacterial 16S rRNA gene.

ABSTRACT: The composition of mammalian intestinal microflora is related to many environmental and geographical factors, and it plays an important role in many aspects such as growth and development. Sequencing data of the bacterial 16S rRNA gene from sable (Martes zibellina) samples using next-generation sequencing technology are limited. In our research, 84,116 reads obtained by high-throughput sequencing were analyzed to characterize and compare the intestinal microflora of wild sables and housed sables. Firmicutes (31.1 %), Bacteroidetes (26.0 %) and Proteobacteria (21.5 %) were the three most abundant phyla present in wild sables, whereas Firmicutes (55.6 %), Proteobacteria (29.1 %) and Actinobacteria (6.0 %) were the three predominant phyla present in housed sables. At the phylum level, wild sables exhibited a significant difference in the relative abundances of Bacteroidetes and Actinobacteria, whereas housed sables only exhibited significant changes in TM7 at the phylum level, and Clostridia, at the class level. The predominance of Bacteroidetes in wild sables warrants further research. These results indicate that a sudden change in diet may be a key factor that influences fecal bacterial diversity in mammals.

SUBMITTER: Guan Y

PROVIDER: S-EPMC5061668 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature

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Comparison of the bacterial communities in feces from wild versus housed sables (Martes zibellina) by high-throughput sequence analysis of the bacterial 16S rRNA gene.

Guan Yu Y Zhang Honghai H Gao Xiaodong X Shang Shuai S Wu Xiaoyang X Chen Jun J Zhang Wei W Zhang Weihua W Jiang Mingsheng M Zhang Baohong B Chen Peng P

AMB Express 20161013 1

The composition of mammalian intestinal microflora is related to many environmental and geographical factors, and it plays an important role in many aspects such as growth and development. Sequencing data of the bacterial 16S rRNA gene from sable (Martes zibellina) samples using next-generation sequencing technology are limited. In our research, 84,116 reads obtained by high-throughput sequencing were analyzed to characterize and compare the intestinal microflora of wild sables and housed sables ...[more]

PMID: 27734418

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Project description:Sourdoughs (SDs) are spontaneously formed microbial ecosystems composed of various species of lactic acid bacteria (LAB) and acid-tolerant yeasts in food matrices of cereal flours mixed with water. To date, more than 90 LAB species have been isolated, significantly impacting the organoleptic characteristics, shelf life, and health properties of bakery products. To learn more about the unique bacterial communities involved in creating regional Bulgarian sourdoughs, we examined the metacommunities of five sourdoughs produced by spontaneous fermentation and maintained by backslopping in bakeries from three geographic locations. The 16S rRNA gene amplicon sequencing showed that the former genus Lactobacillus was predominant in the studied sourdoughs (51.0-78.9%). Weissella (0.9-42.8%), Herbaspirillum (1.6-3.8%), Serratia (0.1-11.7%), Pediococcus (0.2-7.5%), Bacteroides (0.1-1.3%), and Sphingomonas (0.1-0.5%) were also found in all 5 samples. Genera Leuconostoc, Enterococcus, Bacillus, and Asaia were sample-specific. It is interesting to note that the genus Weissella was more abundant in wholegrain samples. The greatest diversity at the species level was found in the former genus Lactobacillus, presented in the sourdough samples with 13 species. The UPGMA cluster analysis clearly demonstrated similarity in species' relative abundance between samples from the same location. In addition, we can conclude that the presence of two main clusters-one including samples from mountainous places (the cities of Smolyan and Bansko) and the other including samples from the city of Ruse (the banks of the Danube River)-may indicate the impact of climate and geographic location (e.g., terrain, elevation, land use, and nearby water bodies and their streams) on the abundance of microbiome taxa. As the bacterial population is crucial for bread standardization, we expect the local bakery sector to be interested in the relationship between process variables and their effect on bacterial dynamics described in this research study.

Dataset Information

Comparison of the bacterial communities in feces from wild versus housed sables (Martes zibellina) by high-throughput sequence analysis of the bacterial 16S rRNA gene.

Publications

Comparison of the bacterial communities in feces from wild versus housed sables (Martes zibellina) by high-throughput sequence analysis of the bacterial 16S rRNA gene.

Similar Datasets

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