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Fusarium tricinctum, An Endophytic Fungus Exhibits Cell Growth Inhibition and Antioxidant Activity.

ABSTRACT: An endophytic fungus (strain T6) isolated from Taxus baccata was studied for its effect on the growth of human breast cancer cell line (MCF-7), human cervical cancer cell line (HeLa) and peripheral blood mononuclear cells (PBMCs) as well as for its antioxidant activity. Based on morphological characters and internal transcribed spacer (ITS) sequence analysis, this fungus (strain T6) was identified as Fusarium tricinctum. This fungus has shown inhibition in the growth of the MCF-7 and HeLa cancer cell lines. IC50 values of the fungal extract were 225 ± 26 and 220 ± 18 ?g ml-1 for MCF-7 and HeLa cell lines, respectively. Further, F. tricinctum showed inhibition in the proliferation of concanavalin A stimulated PBMCs indicating its immunosuppressive potential (IC50 value 110 ± 44 ?g ml-1). Tumour necrosis factor (TNF)-? production in concanavalin A stimulated PBMCs and MCF-7 were found to be inhibited which indicates that the antiproliferative effect may be associated with TNF-?. Free radical scavenging results revealed that this fungus also exhibited antioxidant activity (IC50 value 482 ± 9 ?g ml-1). Present study results suggested that F. tricinctum has the potential to be used for therapeutic purposes because of its antiproliferative and antioxidant potential.

SUBMITTER: Vasundhara M

PROVIDER: S-EPMC5061693 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature

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Fusarium tricinctum, An Endophytic Fungus Exhibits Cell Growth Inhibition and Antioxidant Activity.

Vasundhara M M Baranwal Manoj M Kumar Anil A

Indian journal of microbiology 20160524 4

An endophytic fungus (strain T6) isolated from Taxus baccata was studied for its effect on the growth of human breast cancer cell line (MCF-7), human cervical cancer cell line (HeLa) and peripheral blood mononuclear cells (PBMCs) as well as for its antioxidant activity. Based on morphological characters and internal transcribed spacer (ITS) sequence analysis, this fungus (strain T6) was identified as Fusarium tricinctum. This fungus has shown inhibition in the growth of the MCF-7 a ...[more]

PMID: 27784939

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Project description:Glioblastoma is the most devastating primary brain tumor. Current treatment is palliative, making necessary the development of new therapeutic strategies to offer alternatives to patients. Therefore, endophytes represent an interesting source of natural metabolites with anticancer potential. These microorganisms reside in tissues of living plants and act to improve their growth. Evidence revealed that several medicinal plants are colonized by endophytic fungi producer of antitumor metabolites. Achyrocline satureioides is a Brazilian medicinal plant characterized by its properties against gastrointestinal disturbances, anticancer and antioxidant effects. However, there are no reports describing the endophytic composition of A. satureioides. The present study proposes the isolation of endophytic fungus from A. satureioides, extract preparation, phytochemical characterization and evaluation of its antiglioma potential. Our data showed that crude extracts of endophyte decreased glioma viability with IC50 values of 1.60-1.63 μg/mL to eDCM (dichloromethane extract) and 37.30-55.12 μg/mL to eEtAc (ethyl acetate extract), respectively. Crude extracts induced cell death by apoptosis with modulation of redox status. In order to bioprospect anticancer metabolites, endophytic fungus extracts were subjected to guided fractionation and purification yielded five fractions of each extract. Six of ten fractions showed selective antiproliferative activity against glioma cells, with IC50 values ranged from 0.95 to 131.3 μg/mL. F3DCM (from eDCM) and F3EtAc (from eEtAc) fractions promoted C6 glioma toxicity with IC50 of 1.0 and 27.05 μg/mL, respectively. F3EtAc fraction induced late apoptosis and arrest in G2/M stage, while F3DCM promoted apoptosis with arrest in Sub-G1 phase. Moreover, F3DCM increased antioxidant defense and decreased ROS production. Additionally, F3DCM showed no cytotoxic activity against astrocytes, revealing selective effect. Based on promising potential of F3DCM, we identified the production of Sch-642305, a lactone, which showed antiproliferative properties with IC50 values of 1.1 and 7.6 μg/mL to C6 and U138MG gliomas, respectively. Sch-642305 promoted arrest on cell cycle in G2/M inducing apoptosis. Furthermore, this lactone decreased glioma cell migration and modulated redox status, increasing superoxide dismutase and catalase activities and enhancing sulfhydryl content, consequently suppressing reactive species of oxygen generation. Taken together, these results indicate that metabolites produced by endophytic fungus isolated from A. satureioides have therapeutic potential as antiglioma agent.

Dataset Information

Fusarium tricinctum, An Endophytic Fungus Exhibits Cell Growth Inhibition and Antioxidant Activity.

Publications

<i>Fusarium tricinctum</i>, An Endophytic Fungus Exhibits Cell Growth Inhibition and Antioxidant Activity.

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