Dataset Information

Summer Abundance and Distribution of Proteorhodopsin Genes in the Western Arctic Ocean.

ABSTRACT: Proteorhodopsins (PR) are phylogenetically diverse and highly expressed proton pumps in marine bacterial communities. The phylogenetic diversity and in situ expression of the main PR groups in polar off-shore, coastal and estuarine waters is poorly known and their abundance has not yet been reported. Here, we show that PR gene sequences of the southern Beaufort Sea including MacKenzie shelf and estuary are mainly affiliated to Gammaproteobacteria, Alphaproteobacteria, and Bacteroidetes. Substantial overlap (78%) between DNA- and cDNA-based librairies indicated in situ PR transcription within a large fraction of PR-containing community. Sets of specific qPCR primers were designed to measure the absolute abundances of the major PR types. Spatial and depth profiles showed that PR-containing bacteria were abundant throughout the photic zone, comprising up to 45% of total bacteria. Although their abundance varied greatly with location and depth, Alphaproteobacteria predominated in the PR community in all water masses, with SAR11 as the major PR type. Low nutrient concentrations rather than light were the environmental drivers that best explained the abundance and distribution of arctic PR types. Together, our data suggests that PR-based phototrophy could be the major phototrophic prokaryotic process during the Arctic Ocean summer.

SUBMITTER: Boeuf D

PROVIDER: S-EPMC5061748 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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Summer Abundance and Distribution of Proteorhodopsin Genes in the Western Arctic Ocean.

Boeuf Dominique D Lami Raphaël R Cunnington Emelyne E Jeanthon Christian C

Frontiers in microbiology 20161013

Proteorhodopsins (PR) are phylogenetically diverse and highly expressed proton pumps in marine bacterial communities. The phylogenetic diversity and in situ expression of the main PR groups in polar off-shore, coastal and estuarine waters is poorly known and their abundance has not yet been reported. Here, we show that PR gene sequences of the southern Beaufort Sea including MacKenzie shelf and estuary are mainly affiliated to Gammaproteobacteria, Alphaproteobacteria, and Bacter ...[more]

PMID: 27790192

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Project description:Zooplankton plays a pivotal role in linking primary production to higher level consumers in the food webs of marine ecosystems. The distribution of zooplankton is affected by general water conditions, monsoons, currents, and spatial and temporal factors. In the Arctic Ocean, the sea surface is naturally covered with ice. Under ice, water masses interplay to create complex environments that facilitate the transport and distribution of zooplankton, thus altering community structures at geospatial and vertical scales. The present study investigated the species composition and copepod community structures by using geospatial and multiple depth scales, and using multivariate analyses to evaluate the relation of sampling stations and layers. During July-August 2010, zooplankton samples were collected and the temperature and salinity of seawater measured from three stations in the Canada Basin and two stations in the Makarov Basin of the Arctic Ocean (maximum distance of approximately 1400 km). A total of 55 copepod species (including 25 species that were solely identified to the generic level) and 7 taxa of copepodites, altogether belonging to 28 genera, 11 families, and 2 orders were identified, and significant differences were detected in copepod community structures among sampling strata and at geospatial scales. Numerically, Calanus hyperboreus, Calanus copepodite, Calanoida copepodite, Calanus glacialis, and Metridia longa were the most dominant species and taxa. At the local scale, copepod compositions responded differently at each of the sampling stations. At the geospatial scale, the distance between stations MS03 and ICE explained variations in the pattern of dominant species and of copepod community richness. Our study demonstrated varied spatial distribution which indicates that (1) the abundance of copepods at 0-200 m was significantly higher than at other strata, (2) vertical strata affected the distribution of copepod communities, and (3) the interplay of North Pacific and Atlantic waters shaping the copepod assemblage structure at geospatial scales in the Arctic Ocean. The results of our research provide base data for Arctic zooplankton biodiversity and biogeographic distribution.

Dataset Information

Summer Abundance and Distribution of Proteorhodopsin Genes in the Western Arctic Ocean.

Publications

Summer Abundance and Distribution of Proteorhodopsin Genes in the Western Arctic Ocean.

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