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RecA stimulates AlkB-mediated direct repair of DNA adducts.

ABSTRACT: The Escherichia coli AlkB protein is a 2-oxoglutarate/Fe(II)-dependent demethylase that repairs alkylated single stranded and double stranded DNA. Immunoaffinity chromatography coupled with mass spectrometry identified RecA, a key factor in homologous recombination, as an AlkB-associated protein. The interaction between AlkB and RecA was validated by yeast two-hybrid assay; size-exclusion chromatography and standard pull down experiment and was shown to be direct and mediated by the N-terminal domain of RecA. RecA binding results AlkB-RecA heterodimer formation and RecA-AlkB repairs alkylated DNA with higher efficiency than AlkB alone.

SUBMITTER: Shivange G

PROVIDER: S-EPMC5062977 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature

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RecA stimulates AlkB-mediated direct repair of DNA adducts.

Shivange Gururaj G Monisha Mohan M Nigam Richa R Kodipelli Naveena N Anindya Roy R

Nucleic acids research 20160704 18

The Escherichia coli AlkB protein is a 2-oxoglutarate/Fe(II)-dependent demethylase that repairs alkylated single stranded and double stranded DNA. Immunoaffinity chromatography coupled with mass spectrometry identified RecA, a key factor in homologous recombination, as an AlkB-associated protein. The interaction between AlkB and RecA was validated by yeast two-hybrid assay; size-exclusion chromatography and standard pull down experiment and was shown to be direct and mediated by the N-terminal d ...[more]

PMID: 27378775

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