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Localized force application reveals mechanically sensitive domains of Piezo1.

ABSTRACT: Piezos are mechanically activated ion channels that function as sensors of touch and pressure in various cell types. However, the precise mechanism and structures mediating mechanical activation and subsequent inactivation have not yet been identified. Here we use magnetic nanoparticles as localized transducers of mechanical force in combination with pressure-clamp electrophysiology to identify mechanically sensitive domains important for activation and inactivation.

SUBMITTER: Wu J

PROVIDER: S-EPMC5063965 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature

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Localized force application reveals mechanically sensitive domains of Piezo1.

Wu Jason J Goyal Raman R Grandl Jörg J

Nature communications 20161003

Piezos are mechanically activated ion channels that function as sensors of touch and pressure in various cell types. However, the precise mechanism and structures mediating mechanical activation and subsequent inactivation have not yet been identified. Here we use magnetic nanoparticles as localized transducers of mechanical force in combination with pressure-clamp electrophysiology to identify mechanically sensitive domains important for activation and inactivation. ...[more]

PMID: 27694883

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