Dataset Information

Kojic Acid Peptide: A New Compound with Anti-Tyrosinase Potential.

ABSTRACT:

Background

Kojic acid was used for decades in the cosmetic industry as an antimelanogenic agent. However, there are two major drawbacks of Kojic acid, one is cytotoxicity and second are instability on storage. These limitations led the scientist to synthesize the active Kojic acid peptides.

Objective

In the present study, we synthesize and investigate the effect of five Kojic acid peptides to overcome the limitation of Kojic acid.

Methods

The peptide was analyzed and purified by high-performance liquid chromatography and matrix-assisted laser desorption ionization time of flight mass spectroscopy. Further, the tyrosinase activities of the Kojic acid and Kojic acid peptides were compared. The toxicity was measured and the melanin content is recorded in B16F10 mouse melanoma cells.

Results

Maximum tyrosinase activity was measured by Kojic acid peptides. Therefore, Kojic acid peptides were subjected to melanin assay and cytotoxicity assay and finally the stability of the Kojic acid peptide was measured.

Conclusion

It was observed that this newly synthesized Kojic acid peptide is stable and potent to inhibit the tyrosinase activity and melanin content of B16F10 mouse melanoma cells without exhibiting cell toxicity. Together, these preliminary results suggest that a further exploration is being needed to establish Kojic acid peptide as antimelanogenic agent.

SUBMITTER: Singh BK

PROVIDER: S-EPMC5064183 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature

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Publications

Kojic Acid Peptide: A New Compound with Anti-Tyrosinase Potential.

Singh Birendra Kumar BK Park Seok Hoon SH Lee Hyang-Bok HB Goo Young-Aae YA Kim Hyoung Shik HS Cho Seung Hee SH Lee Jeong Hun JH Ahn Ghe Whan GW Kim Jin Pyo JP Kang Su Myoung SM Kim Eun-Ki EK

Annals of dermatology 20160930 5

<h4>Background</h4>Kojic acid was used for decades in the cosmetic industry as an antimelanogenic agent. However, there are two major drawbacks of Kojic acid, one is cytotoxicity and second are instability on storage. These limitations led the scientist to synthesize the active Kojic acid peptides.<h4>Objective</h4>In the present study, we synthesize and investigate the effect of five Kojic acid peptides to overcome the limitation of Kojic acid.<h4>Methods</h4>The peptide was analyzed and purifi ...[more]

PMID: 27746633

Similar Datasets

Project description:Kojic acid, β-arbutin, α-arbutin, and deoxyarbutin have been reported as tyrosinase inhibitors in many articles, but some contradictions exist in their differing results. In order to provide some explanations for these contradictions and to find the most suitable compound as a positive control for screening potential tyrosinase inhibitors, the activity and inhibition type of the aforementioned compounds on monophenolase and diphenolase of mushroom tyrosinase (MTYR) were studied. Their effects on B16F10 cells melanin content, tyrosinase (BTYR) activity, and cell viability were also exposed. Results indicated that α-arbutin competitively inhibited monophenolase activity, whereas they uncompetitively activated diphenolase activity of MTYR. β-arbutin noncompetitively and competitively inhibited monophenolase activity at high molarity (4000 µM) and moderate molarity (250-1000 µM) respectively, whereas it activated the diphenolase activity of MTYR. Deoxyarbutin competitively inhibited diphenolase activity, but could not inhibit monophenolase activity and only extended the lag time. Kojic acid competitively inhibited monophenolase activity and competitive-noncompetitive mixed-type inhibited diphenolase activity of MTYR. In a cellular experiment, deoxyarbutin effectively inhibited BTYR activity and reduced melanin content, but it also potently decreased cell viability. α-arbutin and β-arbutin dose-dependently inhibited BTYR activity, reduced melanin content, and increased cell viability. Kojic acid did not affect cell viability at 43.8-700 µM, but inhibited BTYR activity and reduced melanin content in a dose-dependent manner. Therefore, kojic acid was considered as the most suitable positive control among these four compounds, because it could inhibit both monophenolase and diphenolase activity of MTYR and reduce intercellular melanin content by inhibiting BTYR activity without cytotoxicity. Some explanations for the contradictions in the reported articles were provided.

Dataset Information

Kojic Acid Peptide: A New Compound with Anti-Tyrosinase Potential.

Background

Objective

Methods

Results

Conclusion

Publications

Kojic Acid Peptide: A New Compound with Anti-Tyrosinase Potential.

Similar Datasets

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