Ontology highlight
ABSTRACT:
SUBMITTER: Ferreira BL
PROVIDER: S-EPMC5064609 | biostudies-literature | 2016 Jun
REPOSITORIES: biostudies-literature
Cellular microbiology 20160105 6
Although imaging the live Trypanosoma cruzi parasite is a routine technique in most laboratories, identification of the parasite in infected tissues and organs has been hindered by their intrinsic opaque nature. We describe a simple method for in vivo observation of live single-cell Trypanosoma cruzi parasites inside mammalian host tissues. BALB/c or C57BL/6 mice infected with DsRed-CL or GFP-G trypomastigotes had their organs removed and sectioned with surgical blades. Ex vivo organ sections we ...[more]