Increasing of blastocyst rate and gene expression in co-culture of bovine embryos with adult adipose tissue-derived mesenchymal stem cells.
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ABSTRACT:
SUBMITTER: Miranda MS
PROVIDER: S-EPMC5065556 | biostudies-literature | 2016 Oct
REPOSITORIES: biostudies-literature
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Increasing of blastocyst rate and gene expression in co-culture of bovine embryos with adult adipose tissue-derived mesenchymal stem cells.
Miranda Moysés S MS Nascimento Hamilton S HS Costa Mayra P R MP Costa Nathália N NN Brito Karynne N L KN Lopes Cinthia T A CT Santos Simone S D SS Cordeiro Marcela S MS Ohashi Otávio M OM
Journal of assisted reproduction and genetics 20160730 10
<h4>Purpose</h4>Despite advances in the composition of defined embryo culture media, co-culture with somatic cells is still used for bovine in vitro embryo production (IVEP) in many laboratories worldwide. Granulosa cells are most often used for this purpose, although recent work suggests that co-culture with stem cells of adult or embryonic origin or their derived biomaterials may improve mouse, cattle, and pig embryo development.<h4>Materials and methods</h4>In experiment 1, in vitro produced ...[more]
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