Intracellular pH Response to Weak Acid Stress in Individual Vegetative Bacillus subtilis Cells.

ABSTRACT: Intracellular pH (pH_i) critically affects bacterial cell physiology. Hence, a variety of food preservation strategies are aimed at perturbing pH_i homeostasis. Unfortunately, accurate pH_i quantification with existing methods is suboptimal, since measurements are averages across populations of cells, not taking into account interindividual heterogeneity. Yet, physiological heterogeneity in isogenic populations is well known to be responsible for differences in growth and division kinetics of cells in response to external stressors. To assess in this context the behavior of intracellular acidity, we have developed a robust method to quantify pH_i at single-cell levels in Bacillus subtilis Bacilli spoil food, cause disease, and are well known for thei

SUBMITTER: Pandey R 

PROVIDER: S-EPMC5066363 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature