Ontology highlight
ABSTRACT:
SUBMITTER: Wen L
PROVIDER: S-EPMC5067164 | biostudies-literature | 2016 Sep
REPOSITORIES: biostudies-literature
Wen Liuqing L Huang Kenneth K Zheng Yuan Y Liu Yunpeng Y Zhu He H Wang Peng George PG
Bioorganic & medicinal chemistry letters 20160325 17
A two-step enzymatic strategy for the efficient and convenient synthesis of 6-deoxy-l-sorbose was reported herein. In the first reaction step, the isomerization of l-fucose (6-deoxy-l-galactose) to l-fuculose (6-deoxy-l-tagatose) catalyzed by l-fucose isomerase (FucI), and the epimerization of l-fuculose to 6-deoxy-l-sorbose catalyzed by d-tagatose 3-epimerase (DTE) were coupled with the targeted phosphorylation of 6-deoxy-l-sorbose by fructose kinase from human (HK) in a one-pot reaction. The r ...[more]