Unknown

Dataset Information

0

A two-step strategy for the preparation of 6-deoxy-l-sorbose.


ABSTRACT: A two-step enzymatic strategy for the efficient and convenient synthesis of 6-deoxy-l-sorbose was reported herein. In the first reaction step, the isomerization of l-fucose (6-deoxy-l-galactose) to l-fuculose (6-deoxy-l-tagatose) catalyzed by l-fucose isomerase (FucI), and the epimerization of l-fuculose to 6-deoxy-l-sorbose catalyzed by d-tagatose 3-epimerase (DTE) were coupled with the targeted phosphorylation of 6-deoxy-l-sorbose by fructose kinase from human (HK) in a one-pot reaction. The resultant 6-deoxy-l-sorbose 1-phosphate was purified by silver nitrate precipitation method. In the second reaction step, the phosphate group of the 6-deoxy-l-sorbose 1-phosphate was hydrolyzed with acid phosphatase (AphA) to produce 6-deoxy-l-sorbose in 81% yield with regard to l-fucose.

SUBMITTER: Wen L 

PROVIDER: S-EPMC5067164 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature

altmetric image

Publications

A two-step strategy for the preparation of 6-deoxy-l-sorbose.

Wen Liuqing L   Huang Kenneth K   Zheng Yuan Y   Liu Yunpeng Y   Zhu He H   Wang Peng George PG  

Bioorganic & medicinal chemistry letters 20160325 17


A two-step enzymatic strategy for the efficient and convenient synthesis of 6-deoxy-l-sorbose was reported herein. In the first reaction step, the isomerization of l-fucose (6-deoxy-l-galactose) to l-fuculose (6-deoxy-l-tagatose) catalyzed by l-fucose isomerase (FucI), and the epimerization of l-fuculose to 6-deoxy-l-sorbose catalyzed by d-tagatose 3-epimerase (DTE) were coupled with the targeted phosphorylation of 6-deoxy-l-sorbose by fructose kinase from human (HK) in a one-pot reaction. The r  ...[more]

Similar Datasets

| S-EPMC4990140 | biostudies-literature
| S-EPMC113861 | biostudies-literature
| S-EPMC4229100 | biostudies-literature
| S-EPMC6367226 | biostudies-literature
| S-EPMC2519276 | biostudies-literature
| S-EPMC8262353 | biostudies-literature
| S-EPMC6796870 | biostudies-literature
| S-EPMC7917310 | biostudies-literature
| S-EPMC9921352 | biostudies-literature
| S-EPMC1913458 | biostudies-literature