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Increasing dietary oat fibre decreases the permeability of intestinal mucus.

ABSTRACT: This study investigates the influence of the dietary fibre ?-glucan on nutrient composition and mucus permeability. Pigs were fed a standard diet or a diet containing twice the ?-glucan content for 3 days (n?=?5 per group), followed by the collection of small intestinal mucus and tissue samples. Samples of the consumed diets were subjected to in vitro digestion to determine ?-glucan release, nutrient profile and assessment of mucus permeability. In vitro digestion of the diets indicated that 90% of the ?-glucan was released in the proximal small intestine. Measurements of intestinal mucus showed a reduction in permeability to 100?nm latex beads and also lipid from the digested enhanced ?-glucan diet. The data from this study show for the first time that reducing mass transfer of bile and lipid through the intestinal mucus layer may be one way in which this decrease in bile reabsorption by soluble fibre is enabled.

SUBMITTER: Mackie A

PROVIDER: S-EPMC5070621 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature

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Increasing dietary oat fibre decreases the permeability of intestinal mucus.

Mackie Alan A Rigby Neil N Harvey Pascale P Bajka Balazs B

Journal of functional foods 20161001

This study investigates the influence of the dietary fibre β-glucan on nutrient composition and mucus permeability. Pigs were fed a standard diet or a diet containing twice the β-glucan content for 3 days (n = 5 per group), followed by the collection of small intestinal mucus and tissue samples. Samples of the consumed diets were subjected to <i>in vitro</i> digestion to determine β-glucan release, nutrient profile and assessment of mucus permeability. <i>In vitro</i> digestion of the diets indi ...[more]

PMID: 27790292

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Project description:The gastrointestinal mucus layer represents the last barrier between ingested food or orally administered pharmaceuticals and the mucosal epithelium. This complex gel structure plays an important role in the process of small intestinal absorption. It provides protection against hazardous particles such as bacteria but allows the passage of nutrients and drug molecules towards the intestinal epithelium. In scientific research, mucus from animal sources is usually used to simulate difficult-to-obtain human small intestinal mucus for investigating the intramucus transport of drug delivery systems or food nanoparticles. However, there is a lack of evidence the human mucus can be reliably substituted by animal counterparts for human-relevant transport models. In this report, a procedure for collecting human mucus has been described. More importantly, the permeability characteristics of human and porcine small intestinal mucus secretions to sub-micron sized particles have been compared under simulated intestinal conditions. Negatively charged, 500 nm latex beads were used in multiple-particle tracking experiments to examine the heterogeneity and penetrability of mucus from different sources. Diffusion of the probe particles in adult human ileal mucus and adult pig jejunal and ileal mucus revealed no significant differences in microstructural organisation or microviscosity between the three mucus types (P > 0.05). In contrast to this interspecies similarity, the intraspecies comparison of particle diffusivity in the mucus obtained from adult pigs vs. 2-week old piglets showed better penetrability of the piglet mucus. The mean Stokes-Einstein viscosity of the piglet jejunal mucus was approx. two times lower than the viscosity of the pig jejunal mucus (P < 0.05). All mucus structures were also visualised by scanning electron microscopy. This work validates the use of porcine small intestinal mucus collected from fully-grown pigs for studying colloidal transport of sub-micron sized particles in mucus under conditions mimicking the adult human small intestinal environment.

Dataset Information

Increasing dietary oat fibre decreases the permeability of intestinal mucus.

Publications

Increasing dietary oat fibre decreases the permeability of intestinal mucus.

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