Ontology highlight
ABSTRACT:
SUBMITTER: Bernt A
PROVIDER: S-EPMC5073337 | biostudies-literature | 2016 Oct
REPOSITORIES: biostudies-literature
Bernt Alexander A Rangrez Ashraf Y AY Eden Matthias M Jungmann Andreas A Katz Sylvia S Rohr Claudia C Müller Oliver J OJ Katus Hugo A HA Sossalla Samuel T ST Williams Tatjana T Ritter Oliver O Frank Derk D Frey Norbert N
Scientific reports 20161021
The objective of this study was to identify unknown modulators of Calcineurin (Cn)-NFAT signaling. Measurement of NFAT reporter driven luciferase activity was therefore utilized to screen a human cardiac cDNA-library (~10<sup>7</sup> primary clones) in C2C12 cells through serial dilutions until single clones could be identified. This extensive screening strategy culminated in the identification of SUMO2 as a most efficient Cn-NFAT activator. SUMO2-mediated activation of Cn-NFAT signaling in card ...[more]