Transposon-Sequencing Analysis Unveils Novel Genes Involved in the Generation of Persister Cells in Uropathogenic Escherichia coli.
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ABSTRACT:
SUBMITTER: Molina-Quiroz RC
PROVIDER: S-EPMC5075105 | biostudies-literature | 2016 Nov
REPOSITORIES: biostudies-literature
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Transposon-Sequencing Analysis Unveils Novel Genes Involved in the Generation of Persister Cells in Uropathogenic Escherichia coli.
Molina-Quiroz Roberto C RC Lazinski David W DW Camilli Andrew A Levy Stuart B SB
Antimicrobial agents and chemotherapy 20161021 11
Persister cells are highly tolerant to different antibiotics and are associated with relapsing infections. In order to understand this phenomenon further, we exposed a transposon library to a lethal concentration of ampicillin, and mutants that survived were identified by transposon sequencing (Tn-Seq). We determined that mutations related to carbon metabolism, cell envelope (cell wall generation and membrane proteins), and stress response have a role in persister cell generation. ...[more]
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