Dataset Information

Isolation and identification of EST-SSR markers in Chunia bucklandioides (Hamamelidaceae).

ABSTRACT: PREMISE OF THE STUDY:Chunia bucklandioides (Hamamelidaceae), endemic to Hainan, China, is listed as threatened in the IUCN Red List and is now only found on Mt. Diaoluo and Mt. Jianfeng. Thus, microsatellite markers were developed for future conservation genetic studies of this species. METHODS AND RESULTS:A total of 115 primers were designed on the basis of the transcriptome data of C. bucklandioides. Of them, 59 successfully amplified in C. bucklandioides and polymorphisms were detected in 11; the number of alleles per locus varied from two to five, the observed heterozygosity ranged from 0.000 to 0.941, and the expected heterozygosity ranged from 0.000 to 0.699. A total of 13 primers amplified in Mytilaria laosensis, and five primers amplified in Exbucklandia tonkinensis and E. populnea. CONCLUSIONS:The markers screened here provide a basis to assess genetic structure and further establish conservation strategies for C. bucklandioides.

SUBMITTER: Meng K

PROVIDER: S-EPMC5077281 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature

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Isolation and identification of EST-SSR markers in <i>Chunia bucklandioides</i> (Hamamelidaceae).

Meng Kaikai K Li Mingwan M Fan Qiang Q Tan Weizheng W Sun Jian J Liao Wenbo W Chen Sufang S

Applications in plant sciences 20161014 10

<h4>Premise of the study</h4><i>Chunia bucklandioides</i> (Hamamelidaceae), endemic to Hainan, China, is listed as threatened in the IUCN Red List and is now only found on Mt. Diaoluo and Mt. Jianfeng. Thus, microsatellite markers were developed for future conservation genetic studies of this species.<h4>Methods and results</h4>A total of 115 primers were designed on the basis of the transcriptome data of <i>C. bucklandioides</i>. Of them, 59 successfully amplified in <i>C. bucklandioides</i> an ...[more]

PMID: 27785382

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Project description:Russian wildrye, Psathyrostachys junceus (Fisch.) Nevski, is widely distributed in the high latitude areas of Eurasia. It plays an important role in grassland ecosystem maintenance, as well as being a valuable palatable forage species for livestock and wildlife. Russian wildrye germplasm has rich phenotypic and genetic diversity and has potential for improvement through crossbreeding. In this study, fifteen Russian wildrye hybrid combinations were produced and one F1 population with 123 putative hybrids was obtained by crossing two individual plants with significant differences in nutritional characteristics and reproductive tiller number. Twelve phenotypic traits of the F1 population were measured for three consecutive years, and ten of the twelve traits were in line with the genetic characteristics of quantitative traits. Hybrid superiority was revealed among F1 hybrids in both nutritional and reproductive traits. One non-recurrent parent plant with the highest PCA-synthesis score was selected and used to make a backcross with the 'BOZOISKY SELECT' male parent, and 143 putative BC1 hybrids were obtained. Sixteen pairs of EST-SSR primers were randomly selected from polymorphic primers derived from different expressed tiller trait related genes. Three primer pairs that amplified both the paternal and maternal characteristic band were used to assess the purity of the F1 population, and three primer pairs (with one shared primer pair) were used to identify the BC1 population. The hybrid purity was 96.75% for the F1 population and 95.80% for the BC1 population, and the results were confirmed by self-fertility test through bagging isolation. The genetic similarity coefficients between the F1 progeny and the male parent ranged from 0.500 to 0.895, and those between the BC1 progeny and the male parent ranged from 0.667 to 0.939. A subset of individuals in the BC1 population had closer genetic distance to the recurrent parent, and genetic variation within the BC1 population decreased compared to the F1 population.

Dataset Information

Isolation and identification of EST-SSR markers in Chunia bucklandioides (Hamamelidaceae).

Publications

Isolation and identification of EST-SSR markers in <i>Chunia bucklandioides</i> (Hamamelidaceae).

Similar Datasets

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