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Massively parallel delivery of large cargo into mammalian cells with light pulses.

ABSTRACT: We report a high-throughput platform for delivering large cargo elements into 100,000 cells in 1 min. Our biophotonic laser-assisted surgery tool (BLAST) generates an array of microcavitation bubbles that explode in response to laser pulsing, forming pores in adjacent cell membranes through which cargo is gently driven by pressurized flow. The platform delivers large items including bacteria, enzymes, antibodies and nanoparticles into diverse cell types with high efficiency and cell viability. We used this platform to explore the intracellular lifestyle of Francisella novicida and discovered that the iglC gene is unexpectedly required for intracellular replication even after phagosome escape into the cell cytosol.

SUBMITTER: Wu YC

PROVIDER: S-EPMC5082232 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature

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Massively parallel delivery of large cargo into mammalian cells with light pulses.

Wu Yi-Chien YC Wu Ting-Hsiang TH Clemens Daniel L DL Lee Bai-Yu BY Wen Ximiao X Horwitz Marcus A MA Teitell Michael A MA Chiou Pei-Yu PY

Nature methods 20150406 5

We report a high-throughput platform for delivering large cargo elements into 100,000 cells in 1 min. Our biophotonic laser-assisted surgery tool (BLAST) generates an array of microcavitation bubbles that explode in response to laser pulsing, forming pores in adjacent cell membranes through which cargo is gently driven by pressurized flow. The platform delivers large items including bacteria, enzymes, antibodies and nanoparticles into diverse cell types with high efficiency and cell viability. W ...[more]

PMID: 25849636

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