Ontology highlight
ABSTRACT:
SUBMITTER: Rabe P
PROVIDER: S-EPMC5082573 | biostudies-literature | 2016
REPOSITORIES: biostudies-literature
Rabe Patrick P Schmitz Thomas T Dickschat Jeroen S JS
Beilstein journal of organic chemistry 20160815
The products obtained by incubation of farnesyl diphosphate (FPP) with six purified bacterial terpene cyclases were characterised by one- and two-dimensional NMR spectroscopic methods, allowing for a full structure elucidation. The absolute configurations of four terpenes were determined based on their optical rotary powers. Incubation experiments with <sup>13</sup>C-labelled isotopomers of FPP in buffers containing water or deuterium oxide allowed for detailed insights into the cyclisation mech ...[more]