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Mechanistic investigations on six bacterial terpene cyclases.

ABSTRACT: The products obtained by incubation of farnesyl diphosphate (FPP) with six purified bacterial terpene cyclases were characterised by one- and two-dimensional NMR spectroscopic methods, allowing for a full structure elucidation. The absolute configurations of four terpenes were determined based on their optical rotary powers. Incubation experiments with ¹³C-labelled isotopomers of FPP in buffers containing water or deuterium oxide allowed for detailed insights into the cyclisation mechanisms of the bacterial terpene cyclases.

SUBMITTER: Rabe P

PROVIDER: S-EPMC5082573 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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Mechanistic investigations on six bacterial terpene cyclases.

Rabe Patrick P Schmitz Thomas T Dickschat Jeroen S JS

Beilstein journal of organic chemistry 20160815

The products obtained by incubation of farnesyl diphosphate (FPP) with six purified bacterial terpene cyclases were characterised by one- and two-dimensional NMR spectroscopic methods, allowing for a full structure elucidation. The absolute configurations of four terpenes were determined based on their optical rotary powers. Incubation experiments with <sup>13</sup>C-labelled isotopomers of FPP in buffers containing water or deuterium oxide allowed for detailed insights into the cyclisation mech ...[more]

PMID: 27829890

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