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Mechanistic investigations on six bacterial terpene cyclases.


ABSTRACT: The products obtained by incubation of farnesyl diphosphate (FPP) with six purified bacterial terpene cyclases were characterised by one- and two-dimensional NMR spectroscopic methods, allowing for a full structure elucidation. The absolute configurations of four terpenes were determined based on their optical rotary powers. Incubation experiments with 13C-labelled isotopomers of FPP in buffers containing water or deuterium oxide allowed for detailed insights into the cyclisation mechanisms of the bacterial terpene cyclases.

SUBMITTER: Rabe P 

PROVIDER: S-EPMC5082573 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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Mechanistic investigations on six bacterial terpene cyclases.

Rabe Patrick P   Schmitz Thomas T   Dickschat Jeroen S JS  

Beilstein journal of organic chemistry 20160815


The products obtained by incubation of farnesyl diphosphate (FPP) with six purified bacterial terpene cyclases were characterised by one- and two-dimensional NMR spectroscopic methods, allowing for a full structure elucidation. The absolute configurations of four terpenes were determined based on their optical rotary powers. Incubation experiments with <sup>13</sup>C-labelled isotopomers of FPP in buffers containing water or deuterium oxide allowed for detailed insights into the cyclisation mech  ...[more]

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