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N-Glycopeptide Profiling in Arabidopsis Inflorescence.

ABSTRACT: This study presents the first large-scale analysis of plant intact glycopeptides. Using wheat germ agglutinin lectin weak affinity chromatography to enrich modified peptides, followed by electron transfer dissociation (ETD)(1) fragmentation tandem mass spectrometry, glycan compositions on over 1100 glycopeptides from 270 proteins found in Arabidopsis inflorescence tissue were characterized. While some sites were only detected with a single glycan attached, others displayed up to 16 different glycoforms. Among the identified glycopeptides were four modified in nonconsensus glycosylation motifs. While most of the modified proteins are secreted, membrane, endoplasmic reticulum (ER), or Golgi-localized proteins, surprisingly, N-linked sugars were detected on a protein predicted to be cytosolic or nuclear.

SUBMITTER: Xu SL

PROVIDER: S-EPMC5083099 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature

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N-Glycopeptide Profiling in Arabidopsis Inflorescence.

Xu Shou-Ling SL Medzihradszky Katalin F KF Wang Zhi-Yong ZY Burlingame Alma L AL Chalkley Robert J RJ

Molecular & cellular proteomics : MCP 20160411 6

This study presents the first large-scale analysis of plant intact glycopeptides. Using wheat germ agglutinin lectin weak affinity chromatography to enrich modified peptides, followed by electron transfer dissociation (ETD)(1) fragmentation tandem mass spectrometry, glycan compositions on over 1100 glycopeptides from 270 proteins found in Arabidopsis inflorescence tissue were characterized. While some sites were only detected with a single glycan attached, others displayed up to 16 different gly ...[more]

PMID: 27067053

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