High-resolution microbiota flow cytometry reveals dynamic colitis-associated changes in fecal bacterial composition.
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ABSTRACT:
SUBMITTER: Zimmermann J
PROVIDER: S-EPMC5084791 | biostudies-literature | 2016 May
REPOSITORIES: biostudies-literature
Publications
High-resolution microbiota flow cytometry reveals dynamic colitis-associated changes in fecal bacterial composition.
Zimmermann Jakob J Hübschmann Thomas T Schattenberg Florian F Schumann Joachim J Durek Pawel P Riedel René R Friedrich Marie M Glauben Rainer R Siegmund Britta B Radbruch Andreas A Müller Susann S Chang Hyun-Dong HD
European journal of immunology 20160501 5
Using high-resolution flow cytometry of bacterial shape (forward scatter) and DNA content (DAPI staining), we detected dramatic differences in the fecal microbiota composition during murine colitis that were validated using 16S rDNA sequencing. This innovative method provides a fast and inexpensive tool to interrogate the microbiota on the single-cell level. ...[more]
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