Neoadjuvant tamoxifen synchronizes ER? binding and gene expression profiles related to outcome and proliferation.

ABSTRACT: Estrogen receptor alpha (ER?)-positive breast cancers are frequently treated with tamoxifen, but resistance is common. It remains elusive how tamoxifen resistance occurs and predictive biomarkers for treatment outcome are needed. Because most biomarker discovery studies are performed using pre-treatment surgical resections, the effects of tamoxifen therapy directly on the tumor cell in vivo remain unexamined. In this study, we assessed DNA copy number, gene expression profiles and ER?/chromatin binding landscapes on breast tumor specimens, both before and after neoadjuvant tamoxifen treatment. We observed neoadjuvant tamoxifen treatment synchronized ER?/chromatin interactions and downstream gene expression, indicating that hormonal therapy reduces inter-tumor molecular variability. ER?-synchronized sites are associated with dynamic FOXA1 action at these sites, which is under control of growth factor signaling. Genes associated with tamoxifen-synchronized sites are capable of differentiating patients for tamoxifen benefit. Due to the direct effects of therapeutics on ER? behavior and transcriptional output, our study highlights the added value of biomarker discovery studies after neoadjuvant drug exposure.

SUBMITTER: Severson TM 

PROVIDER: S-EPMC5085127 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature