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Direct LAMP Assay without Prior DNA Purification for Sex Determination of Papaya.


ABSTRACT: Papaya (Carica papaya L.) is an economically important tropical fruit tree with hermaphrodite, male and female sex types. Hermaphroditic plants are the major type used for papaya production because their fruits have more commercial advantages than those of female plants. Sex determination of the seedlings, or during the early growth stages, is very important for the papaya seedling industry. Thus far, the only method for determining the sex type of a papaya at the seedling stage has been DNA analysis. In this study, a molecular technique-based on DNA analysis-was developed for detecting male-hermaphrodite-specific markers to examine the papaya's sex type. This method is based on the loop-mediated isothermal amplification (LAMP) and does not require prior DNA purification. The results show that the method is an easy, efficient, and inexpensive way to determine a papaya's sex. This is the first report on the LAMP assay, using intact plant materials-without DNA purification-as samples for the analysis of sex determination of papaya. We found that using high-efficiency DNA polymerase was essential for successful DNA amplification, using trace intact plant material as a template DNA source.

SUBMITTER: Tsai CC 

PROVIDER: S-EPMC5085663 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature

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Direct LAMP Assay without Prior DNA Purification for Sex Determination of Papaya.

Tsai Chi-Chu CC   Shih Huei-Chuan HC   Ko Ya-Zhu YZ   Wang Ren-Huang RH   Li Shu-Ju SJ   Chiang Yu-Chung YC  

International journal of molecular sciences 20160924 10


Papaya (Carica papaya L.) is an economically important tropical fruit tree with hermaphrodite, male and female sex types. Hermaphroditic plants are the major type used for papaya production because their fruits have more commercial advantages than those of female plants. Sex determination of the seedlings, or during the early growth stages, is very important for the papaya seedling industry. Thus far, the only method for determining the sex type of a papaya at the seedling stage has been DNA ana  ...[more]

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