Assessment of 'one-step' versus 'sequential' embryo culture conditions through embryonic genome methylation and hydroxymethylation changes.
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ABSTRACT:
SUBMITTER: Salvaing J
PROVIDER: S-EPMC5088634 | biostudies-literature | 2016 Nov
REPOSITORIES: biostudies-literature
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Assessment of 'one-step' versus 'sequential' embryo culture conditions through embryonic genome methylation and hydroxymethylation changes.
Salvaing J J Peynot N N Bedhane M N MN Veniel S S Pellier E E Boulesteix C C Beaujean N N Daniel N N Duranthon V V
Human reproduction (Oxford, England) 20160922 11
<h4>Study question</h4>In comparison to in vivo development, how do different conditions of in vitro culture ('one step' versus 'sequential medium') impact DNA methylation and hydroxymethylation in preimplantation embryos?<h4>Summary answer</h4>Using rabbit as a model, we show that DNA methylation and hydroxymethylation are both affected by in vitro culture of preimplantation embryos and the effect observed depends on the culture medium used.<h4>What is known already</h4>Correct regulation of DN ...[more]
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