Improving virus production through quasispecies genomic selection and molecular breeding.
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ABSTRACT:
SUBMITTER: Perez-Rodriguez FJ
PROVIDER: S-EPMC5093897 | biostudies-literature | 2016 Nov
REPOSITORIES: biostudies-literature
Publications
Improving virus production through quasispecies genomic selection and molecular breeding.
Pérez-Rodríguez Francisco J FJ D'Andrea Lucía L de Castellarnau Montserrat M Costafreda Maria Isabel MI Guix Susana S Ribes Enric E Quer Josep J Gregori Josep J Bosch Albert A Pintó Rosa M RM
Scientific reports 20161103
Virus production still is a challenging issue in antigen manufacture, particularly with slow-growing viruses. Deep-sequencing of genomic regions indicative of efficient replication may be used to identify high-fitness minority individuals suppressed by the ensemble of mutants in a virus quasispecies. Molecular breeding of quasispecies containing colonizer individuals, under regimes allowing more than one replicative cycle, is a strategy to select the fittest competitors among the colonizers. A s ...[more]
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