Ontology highlight
ABSTRACT:
SUBMITTER: Ferrari AR
PROVIDER: S-EPMC5095424 | biostudies-literature | 2016 Nov
REPOSITORIES: biostudies-literature
Ferrari Alessandro R AR Rozeboom Henriëtte J HJ Dobruchowska Justyna M JM van Leeuwen Sander S SS Vugts Aniek S C AS Koetsier Martijn J MJ Visser Jaap J Fraaije Marco W MW
The Journal of biological chemistry 20160914 45
By inspection of the predicted proteome of the fungus Myceliophthora thermophila C1 for vanillyl-alcohol oxidase (VAO)-type flavoprotein oxidases, a putative oligosaccharide oxidase was identified. By homologous expression and subsequent purification, the respective protein could be obtained. The protein was found to contain a bicovalently bound FAD cofactor. By screening a large number of carbohydrates, several mono- and oligosaccharides could be identified as substrates. The enzyme exhibits a ...[more]