Entropic stabilization of folded RNA in crowded solutions measured by SAXS.

ABSTRACT: Non-coding RNAs must fold into specific structures that are stabilized by metal ions and other co-solutes in the cell's interior. Large crowder molecules such as PEG stabilize a bacterial group I ribozyme so that the RNA folds in low Mg²⁺ concentrations typical of the cell's interior. To understand the thermodynamic origins of stabilization by crowder molecules, small angle X-ray scattering was used to measure the folding and helix assembly of a bacterial group I ribozyme at different temperatures and in different MgCl₂ and polyethylene glycol (PEG) concentrations. The resulting phase diagrams show that perturbations to folding by each variable do not overlap. A favorable enthalpy change drives the formation of compact, native-like structures, but requires Mg^2+</

SUBMITTER: Kilburn D 

PROVIDER: S-EPMC5100557 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature