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Paraffin processing of stented arteries using a postfixation dissolution of metallic and polymeric stents.


ABSTRACT: Studying the morphology of the arterial response to endovascular stent implantation requires embedding the explanted stented artery in rigid materials such as poly(methyl methacrylate) to enable sectioning through both the in situ stent and the arterial wall, thus maintaining the proper anatomic relationships. This is a laborious, time-consuming process. Moreover, the technical quality of stained plastic sections is typically suboptimal and, in some cases, precludes immunohistochemical analysis. Here we describe a novel technique for dissolution of metallic and plastic stents that is compatible with subsequent embedding of "destented" arteries in paraffin, fine sectioning, major staining protocols, and immunohistochemistry.

SUBMITTER: Fishbein I 

PROVIDER: S-EPMC5101146 | biostudies-literature | 2016 Nov - Dec

REPOSITORIES: biostudies-literature

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Paraffin processing of stented arteries using a postfixation dissolution of metallic and polymeric stents.

Fishbein Ilia I   Welch Tre T   Guerrero David T DT   Alferiev Ivan S IS   Adamo Richard F RF   Chorny Michael M   Gupte Rohit K RK   Tang Yanqing Y   Levy Robert J RJ  

Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology 20160820 6


Studying the morphology of the arterial response to endovascular stent implantation requires embedding the explanted stented artery in rigid materials such as poly(methyl methacrylate) to enable sectioning through both the in situ stent and the arterial wall, thus maintaining the proper anatomic relationships. This is a laborious, time-consuming process. Moreover, the technical quality of stained plastic sections is typically suboptimal and, in some cases, precludes immunohistochemical analysis.  ...[more]

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