Ontology highlight
ABSTRACT:
SUBMITTER: Jeong YG
PROVIDER: S-EPMC5101302 | biostudies-literature | 2016 Dec
REPOSITORIES: biostudies-literature
Jeong Yun Gyu YG Lee Jin Sil JS Shim Jae Kwon JK Hur Won W
Cytotechnology 20160926 6
Multicellular spheroids are obtained in a variety of three-dimensional (3D) culture systems without the use of supporting scaffold. We present here a 3D culture method that resulted in a multicellular sheet under scaffold-free conditions. A floating disk-shaped 3D culture was prepared by magnetic levitation of B16F10 cells that has ingested Fe<sub>3</sub>O<sub>4</sub>-containing fibroin microspheres. The melanoma disk grew up to 19 mm in diameter and the thickness was ranged between 80 and 100 μ ...[more]