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Chemically Engineered Nanoparticle-Protein Interface for Real-Time Cellular Oxidative Stress Monitoring.

ABSTRACT: A co-engineered nanoparticle/protein peroxide detector is created. This system features a gold nanoparticle functionalized with a galactose headgroup (AuNP-Gal) that reacts covalently with a boronate-modified green fluorescent protein (PB-GFP). Boronate acid-saccharide complexation between PB-GFP and AuNP-Gal affords a highly stable assembly. This complex is disrupted by peroxide, allowing quantitative and selective monitoring of hydrogen peroxide production in real time.

SUBMITTER: Jiang Y 

PROVIDER: S-EPMC5101542 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature

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Chemically Engineered Nanoparticle-Protein Interface for Real-Time Cellular Oxidative Stress Monitoring.

Jiang Ying Y   Wang Ming M   Hardie Joseph J   Tonga Gulen Yesilbag GY   Ray Moumita M   Xu Qiaobing Q   Rotello Vincent M VM  

Small (Weinheim an der Bergstrasse, Germany) 20160613 28

A co-engineered nanoparticle/protein peroxide detector is created. This system features a gold nanoparticle functionalized with a galactose headgroup (AuNP-Gal) that reacts covalently with a boronate-modified green fluorescent protein (PB-GFP). Boronate acid-saccharide complexation between PB-GFP and AuNP-Gal affords a highly stable assembly. This complex is disrupted by peroxide, allowing quantitative and selective monitoring of hydrogen peroxide production in real time. ...[more]

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