MAS ¹H NMR Probes Freezing Point Depression of Water and Liquid-Gel Phase Transitions in Liposomes.

ABSTRACT: The lipid bilayer typical of hydrated biological membranes is characterized by a liquid-crystalline, highly dynamic state. Upon cooling or dehydration, these membranes undergo a cooperative transition to a rigidified, more-ordered, gel phase. This characteristic phase transition is of significant biological and biophysical interest, for instance in studies of freezing-tolerant organisms. Magic-angle-spinning (MAS) solid-state NMR (ssNMR) spectroscopy allows for the detection and characterization of the phase transitions over a wide temperature range. In this study we employ MAS ¹H NMR to probe the phase transitions of both solvent molecules and different hydrated phospholipids, including tetraoleoyl cardiolipin (TOCL) and several phosphatidylcholine lipid species. The employed M

SUBMITTER: Mandal A 

PROVIDER: S-EPMC5103019 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature