Ontology highlight
ABSTRACT:
SUBMITTER: Sikora A
PROVIDER: S-EPMC5103032 | biostudies-literature | 2016 Nov
REPOSITORIES: biostudies-literature
Sikora Arthur A Joseph Benesh B Matson Morgan M Staley Jacob R JR Cafiso David S DS
Biophysical journal 20161101 9
In BtuB, the Escherichia coli TonB-dependent transporter for vitamin B<sub>12</sub>, substrate binding to the extracellular surface unfolds a conserved energy coupling motif termed the Ton box into the periplasm. This transmembrane signaling event facilitates an interaction between BtuB and the inner-membrane protein TonB. In this study, continuous-wave and pulse electron paramagnetic resonance in a native outer-membrane preparation demonstrate that signaling also occurs from the periplasmic to ...[more]