Dynamic Oligomerization of Integrase Orchestrates HIV Nuclear Entry.
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ABSTRACT:
SUBMITTER: Borrenberghs D
PROVIDER: S-EPMC5103197 | biostudies-literature | 2016 Nov
REPOSITORIES: biostudies-literature
Publications
Dynamic Oligomerization of Integrase Orchestrates HIV Nuclear Entry.
Borrenberghs Doortje D Dirix Lieve L De Wit Flore F Rocha Susana S Blokken Jolien J De Houwer Stéphanie S Gijsbers Rik R Christ Frauke F Hofkens Johan J Hendrix Jelle J Debyser Zeger Z
Scientific reports 20161110
Nuclear entry is a selective, dynamic process granting the HIV-1 pre-integration complex (PIC) access to the chromatin. Classical analysis of nuclear entry of heterogeneous viral particles only yields averaged information. We now have employed single-virus fluorescence methods to follow the fate of single viral pre-integration complexes (PICs) during infection by visualizing HIV-1 integrase (IN). Nuclear entry is associated with a reduction in the number of IN molecules in the complexes while th ...[more]
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