Dataset Information

Development and characterization of microsatellite loci for Lindera glauca (Lauraceae).

ABSTRACT: PREMISE OF THE STUDY:Microsatellite primers were developed to investigate population genetic structure in Lindera glauca (Lauraceae). METHODS AND RESULTS:Twenty-five microsatellite primers were developed and optimized for L. glauca using Illumina's Solexa sequencing technology. These novel primers were found to be polymorphic in nine wild L. glauca populations with 81 total alleles confirmed and genotyped via capillary gel electrophoresis. The total number of alleles, observed heterozygosity, and expected heterozygosity for each population ranged from one to four, from 0.00 to 0.90, and from 0.00 to 0.79, respectively. In addition, the 25 primers were tested in 10 additional individuals of the related species L. communis, and all but four markers showed good amplification results. CONCLUSIONS:This set of microsatellite primers is the first specifically developed for L. glauca and will facilitate studies of genetic diversity and evolution among populations of this species.

SUBMITTER: Xiong B

PROVIDER: S-EPMC5104530 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Development and characterization of microsatellite loci for <i>Lindera glauca</i> (Lauraceae).

Xiong Biao B Dong Shubin S Qi Ji J Zhang Limei L Ha Denglong D Ju Yuxi Y Zhang Zhixiang Z

Applications in plant sciences 20161104 11

<h4>Premise of the study</h4>Microsatellite primers were developed to investigate population genetic structure in <i>Lindera glauca</i> (Lauraceae).<h4>Methods and results</h4>Twenty-five microsatellite primers were developed and optimized for <i>L. glauca</i> using Illumina's Solexa sequencing technology. These novel primers were found to be polymorphic in nine wild <i>L. glauca</i> populations with 81 total alleles confirmed and genotyped via capillary gel electrophoresis. The total number of ...[more]

PMID: 27843729

Similar Datasets

Project description:Lindera glauca (Lauraceae) is a tree of economic and ecological significance that reproduces sexually and asexually via apomictic seeds. It is widely distributed in the low-altitude montane forests of East Asia. Despite the potential implications of a mixed reproductive system in terms of genetic diversity, few studies have focused on this aspect. In this study, the genetic structure of wild populations of L. glauca was investigated via genetic analyses. Overall, 13 nuclear microsatellites (nSSRs) and five chloroplast microsatellites (cpSSRs) were used to genotype 300 individual plants, taken from 20 wild populations (a small sample size in some wild populations is due to the limitation of its specific reproduction, leading to certain limitations in the results of this study) and two cultivated populations ranging across nearly the entire natural distribution of mainland China. The populations exhibited low levels of genetic diversity (nSSR: AR = 1.75, Ho = 0.32, He = 0.36; cpSSR: Nb = 2.01, Hrs = 0.40), and no significant effect of isolation by distance between populations existed, regardless of marker type (nSSR: R2 = 0.0401, P = 0.068; cpSSR: R2 = 0.033, P = 0.091). Haplotype networks showed complex relationships among populations, and the H12 haplotype was predominant in most populations. Analyses of molecular variance obtained with nuclear markers (Fsc = 0.293, F ST = 0.362) and chloroplast markers (Fsc = 0.299, F ST = 0.312) were similar. The migration ratio of pollen flow versus seed flow in this study was negative (r = -1.149). Results suggest that weak barriers of dispersal between populations and/or the similarity of founders shared between neighbors and distant populations are indicative of the gene flow between populations more likely involving seeds. Wild L. glauca in mainland China was inferred to have highly skewed sex ratios with predominant females. In addition, some populations experienced a recent bottleneck effect, especially in Gujianshan, Chongqing, and southwest China (population GJS). It is suggested that few wild male individuals should be conserved in order to maintain overall genetic diversity in the wild populations of this species. These findings provide important information for the sustainable utilization and preservation of the overall genetic diversity of L. glauca.

Dataset Information

Development and characterization of microsatellite loci for Lindera glauca (Lauraceae).

Publications

Development and characterization of microsatellite loci for <i>Lindera glauca</i> (Lauraceae).

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure