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Nanoparticle-mediated photothermal effect enables a new method for quantitative biochemical analysis using a thermometer.

ABSTRACT: A new biomolecular quantitation method, nanoparticle-mediated photothermal bioassay, using a common thermometer as the signal reader was developed. Using an immunoassay as a proof of concept, iron oxide nanoparticles (NPs) captured in the sandwich-type assay system were transformed into a near-infrared (NIR) laser-driven photothermal agent, Prussian blue (PB) NPs, which acted as a photothermal probe to convert the assay signal into heat through the photothermal effect, thus allowing sensitive biomolecular quantitation using a thermometer. This is the first report of biomolecular quantitation using a thermometer and also serves as the first attempt to introduce the nanoparticle-mediated photothermal effect for bioassays.

SUBMITTER: Fu G

PROVIDER: S-EPMC5106188 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature

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Nanoparticle-mediated photothermal effect enables a new method for quantitative biochemical analysis using a thermometer.

Fu Guanglei G Sanjay Sharma T ST Dou Maowei M Li XiuJun X

Nanoscale 20160301 10

A new biomolecular quantitation method, nanoparticle-mediated photothermal bioassay, using a common thermometer as the signal reader was developed. Using an immunoassay as a proof of concept, iron oxide nanoparticles (NPs) captured in the sandwich-type assay system were transformed into a near-infrared (NIR) laser-driven photothermal agent, Prussian blue (PB) NPs, which acted as a photothermal probe to convert the assay signal into heat through the photothermal effect, thus allowing sensitive bi ...[more]

PMID: 26838516

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