Unknown

Dataset Information

0

Targeted Differentiation of Regional Ventral Neuroprogenitors and Related Neuronal Subtypes from Human Pluripotent Stem Cells.


ABSTRACT: Embryoid body (EB) formation and adherent culture (AD) paradigms are equivalently thought to be applicable for neural specification of human pluripotent stem cells. Here, we report that sonic hedgehog-induced ventral neuroprogenitors under EB conditions are fated to medial ganglionic eminence (MGE), while the AD cells mostly adopt a floor-plate (FP) fate. The EB-MGE later on differentiates into GABA and cholinergic neurons, while the AD-FP favors dopaminergic neuron specification. Distinct developmental, metabolic, and adhesion traits in AD and EB cells may potentially account for their differential patterning potency. Gene targeting combined with small-molecule screening experiments identified that concomitant inhibition of Wnts, STAT3, and p38 pathways (3i) could largely convert FP to MGE under AD conditions. Thus, differentiation paradigms and signaling regulators can be integrated together to specify distinct neuronal subtypes for studying and treating related neurological diseases, such as epilepsy, Alzheimer's disease, and Parkinson's disease.

SUBMITTER: Chi L 

PROVIDER: S-EPMC5106484 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature

altmetric image

Publications

Targeted Differentiation of Regional Ventral Neuroprogenitors and Related Neuronal Subtypes from Human Pluripotent Stem Cells.

Chi Liankai L   Fan Beibei B   Zhang Kunshan K   Du Yanhua Y   Liu Zhongliang Z   Fang Yujiang Y   Chen Zhenyu Z   Ren Xudong X   Xu Xiangjie X   Jiang Cizhong C   Li Siguang S   Ma Lin L   Gao Liang L   Liu Ling L   Zhang Xiaoqing X  

Stem cell reports 20161006 5


Embryoid body (EB) formation and adherent culture (AD) paradigms are equivalently thought to be applicable for neural specification of human pluripotent stem cells. Here, we report that sonic hedgehog-induced ventral neuroprogenitors under EB conditions are fated to medial ganglionic eminence (MGE), while the AD cells mostly adopt a floor-plate (FP) fate. The EB-MGE later on differentiates into GABA and cholinergic neurons, while the AD-FP favors dopaminergic neuron specification. Distinct devel  ...[more]

Similar Datasets

2016-10-15 | GSE82052 | GEO
| S-EPMC5700195 | biostudies-literature
| S-EPMC3024414 | biostudies-literature
| S-EPMC6016089 | biostudies-other
| S-EPMC6913159 | biostudies-literature
| S-EPMC4876239 | biostudies-literature
| S-EPMC8281298 | biostudies-literature
| S-EPMC7664055 | biostudies-literature
| S-EPMC5984104 | biostudies-literature
| S-EPMC8761391 | biostudies-literature