Ontology highlight
ABSTRACT:
SUBMITTER: Chi L
PROVIDER: S-EPMC5106484 | biostudies-literature | 2016 Nov
REPOSITORIES: biostudies-literature
Chi Liankai L Fan Beibei B Zhang Kunshan K Du Yanhua Y Liu Zhongliang Z Fang Yujiang Y Chen Zhenyu Z Ren Xudong X Xu Xiangjie X Jiang Cizhong C Li Siguang S Ma Lin L Gao Liang L Liu Ling L Zhang Xiaoqing X
Stem cell reports 20161006 5
Embryoid body (EB) formation and adherent culture (AD) paradigms are equivalently thought to be applicable for neural specification of human pluripotent stem cells. Here, we report that sonic hedgehog-induced ventral neuroprogenitors under EB conditions are fated to medial ganglionic eminence (MGE), while the AD cells mostly adopt a floor-plate (FP) fate. The EB-MGE later on differentiates into GABA and cholinergic neurons, while the AD-FP favors dopaminergic neuron specification. Distinct devel ...[more]