High-Throughput Toxicity and Phenotypic Screening of 3D Human Neural Progenitor Cell Cultures on a Microarray Chip Platform.
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ABSTRACT:
SUBMITTER: Nierode GJ
PROVIDER: S-EPMC5106528 | biostudies-literature | 2016 Nov
REPOSITORIES: biostudies-literature
Publications
High-Throughput Toxicity and Phenotypic Screening of 3D Human Neural Progenitor Cell Cultures on a Microarray Chip Platform.
Nierode Gregory J GJ Perea Brian C BC McFarland Sean K SK Pascoal Jorge F JF Clark Douglas S DS Schaffer David V DV Dordick Jonathan S JS
Stem cell reports 20161027 5
A 3D cell culture chip was used for high-throughput screening of a human neural progenitor cell line. The differential toxicity of 24 compounds was determined on undifferentiated and differentiating NPCs. Five compounds led to significant differences in IC<sub>50</sub> values between undifferentiated and differentiating cultures. This platform has potential use in phenotypic screening to elucidate molecular toxicology on human stem cells. ...[more]
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