Ontology highlight
ABSTRACT:
SUBMITTER: Kudirka RA
PROVIDER: S-EPMC5108035 | biostudies-literature | 2016 Nov
REPOSITORIES: biostudies-literature
Kudirka Romas A RA Barfield Robyn M RM McFarland Jesse M JM Drake Penelope M PM Carlson Adam A Bañas Stefanie S Zmolek Wes W Garofalo Albert W AW Rabuka David D
ACS medicinal chemistry letters 20160830 11
Expanded ligation techniques are sorely needed to generate unique linkages for the growing field of functionally enhanced proteins. To address this need, we present a unique chemical ligation that involves the double addition of a pyrazolone moiety with an aldehyde-labeled protein. This ligation occurs via a tandem Knoevenagel condensation-Michael addition. A pyrazolone reacts with an aldehyde to generate an enone, which undergoes subsequent attack by a second pyrazolone to generate a bis-pyrazo ...[more]