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Imaging Cytometry of Human Leukocytes with Third Harmonic Generation Microscopy.

ABSTRACT: Based on third-harmonic-generation (THG) microscopy and a k-means clustering algorithm, we developed a label-free imaging cytometry method to differentiate and determine the types of human leukocytes. According to the size and average intensity of cells in THG images, in a two-dimensional scatter plot, the neutrophils, monocytes, and lymphocytes in peripheral blood samples from healthy volunteers were clustered into three differentiable groups. Using these features in THG images, we could count the number of each of the three leukocyte types both in vitro and in vivo. The THG imaging-based counting results agreed well with conventional blood count results. In the future, we believe that the combination of this THG microscopy-based imaging cytometry approach with advanced texture analysis of sub-cellular features can differentiate and count more types of blood cells with smaller quantities of blood.

SUBMITTER: Wu CH

PROVIDER: S-EPMC5109028 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature

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Imaging Cytometry of Human Leukocytes with Third Harmonic Generation Microscopy.

Wu Cheng-Ham CH Wang Tzung-Dau TD Hsieh Chia-Hung CH Huang Shih-Hung SH Lin Jong-Wei JW Hsu Szu-Chun SC Wu Hau-Tieng HT Wu Yao-Ming YM Liu Tzu-Ming TM

Scientific reports 20161115

Based on third-harmonic-generation (THG) microscopy and a k-means clustering algorithm, we developed a label-free imaging cytometry method to differentiate and determine the types of human leukocytes. According to the size and average intensity of cells in THG images, in a two-dimensional scatter plot, the neutrophils, monocytes, and lymphocytes in peripheral blood samples from healthy volunteers were clustered into three differentiable groups. Using these features in THG images, we could count ...[more]

PMID: 27845443

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