Ontology highlight
ABSTRACT:
SUBMITTER: Wu CH
PROVIDER: S-EPMC5109028 | biostudies-literature | 2016 Nov
REPOSITORIES: biostudies-literature
Wu Cheng-Ham CH Wang Tzung-Dau TD Hsieh Chia-Hung CH Huang Shih-Hung SH Lin Jong-Wei JW Hsu Szu-Chun SC Wu Hau-Tieng HT Wu Yao-Ming YM Liu Tzu-Ming TM
Scientific reports 20161115
Based on third-harmonic-generation (THG) microscopy and a k-means clustering algorithm, we developed a label-free imaging cytometry method to differentiate and determine the types of human leukocytes. According to the size and average intensity of cells in THG images, in a two-dimensional scatter plot, the neutrophils, monocytes, and lymphocytes in peripheral blood samples from healthy volunteers were clustered into three differentiable groups. Using these features in THG images, we could count ...[more]