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Generating iPSC-Derived Choroidal Endothelial Cells to Study Age-Related Macular Degeneration.


ABSTRACT:

Purpose

Age-related macular degeneration (AMD), the most common cause of incurable blindness in the western world, is characterized by the dysfunction and eventual death of choroidal endothelial (CECs), RPE, and photoreceptor cells. Stem cell-based treatment strategies designed to replace photoreceptor and RPE cells currently are a major scientific focus. However, the success of these approaches likely also will require replacement of the underlying, supportive choroidal vasculature. The purpose of this study was to generate stem cell-derived CECs to develop efficient differentiation and transplantation protocols.

Methods

Dermal fibroblasts from the Tie2-GFP mouse were isolated and reprogrammed into two independent induced pluripotent stem cell (iPSC) lines via viral transduction of the transcription factors Oct4, Sox2, Klf4, and c-Myc. Tie2-GFP iPSCs were differentiated into CECs using a coculture method with either the RF6A CEC line or primary mouse CECs. Induced pluripotent stem cell-derived CECs were characterized via RT-PCR and immunocytochemistry for EC- and CEC-specific markers.

Results

Induced pluripotent stem cells generated from mice expressing green fluorescent protein (GFP) under control of the endothelial Tie2 promoter display classic pluripotency markers and stem cell morphology. Induced pluripotent stem cell-derived CECs express carbonic anhydrase IV, eNOS, FOXA2, PLVAP, CD31, CD34, ICAM-1, Tie2, TTR, VE-cadherin, and vWF.

Conclusions

Induced pluripotent stem cell-derived CECs will be a valuable tool for modeling of choriocapillaris-specific insults in AMD and for use in future choroidal endothelial cell replacement approaches.

SUBMITTER: Songstad AE 

PROVIDER: S-EPMC5110238 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature

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Publications

Generating iPSC-Derived Choroidal Endothelial Cells to Study Age-Related Macular Degeneration.

Songstad Allison E AE   Wiley Luke A LA   Duong Khahn K   Kaalberg Emily E   Flamme-Wiese Miles J MJ   Cranston Cathryn M CM   Riker Megan J MJ   Levasseur Dana D   Stone Edwin M EM   Mullins Robert F RF   Tucker Budd A BA  

Investigative ophthalmology & visual science 20151201 13


<h4>Purpose</h4>Age-related macular degeneration (AMD), the most common cause of incurable blindness in the western world, is characterized by the dysfunction and eventual death of choroidal endothelial (CECs), RPE, and photoreceptor cells. Stem cell-based treatment strategies designed to replace photoreceptor and RPE cells currently are a major scientific focus. However, the success of these approaches likely also will require replacement of the underlying, supportive choroidal vasculature. The  ...[more]

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