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Forms and Lability of Phosphorus in Algae and Aquatic Macrophytes Characterized by Solution 31P NMR Coupled with Enzymatic Hydrolysis.

ABSTRACT: Solution Phosphorus-31 nuclear magnetic resonance (31P NMR) spectroscopy coupled with enzymatic hydrolysis (EH) with commercially available phosphatases was used to characterize phosphorus (P) compounds in extracts of the dominant aquatic macrophytes and algae in a eutrophic lake. Total extractable organic P (Po) concentrations ranged from 504 to 1643?mg?kg-1 and 2318 to 8395?mg?kg-1 for aquatic macrophytes and algae, respectively. Using 31P NMR spectroscopy, 11 Po species were detected in the mono- and diester region. Additionally, orthophosphate, pyrophosphate and phosphonates were also detected. Using EH, phytate-like P was identified as the prevalent class of enzyme-labile Po, followed by labile monoester- and diester-P. Comparison of the NMR and EH data indicated that the distribution pattern of major P forms in the samples determined by the two methods was similar (r?=?0.712, p?

SUBMITTER: Feng W

PROVIDER: S-EPMC5111050 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature

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Forms and Lability of Phosphorus in Algae and Aquatic Macrophytes Characterized by Solution 31P NMR Coupled with Enzymatic Hydrolysis.

Feng Weiying W Zhu Yuanrong Y Wu Fengchang F He Zhongqi Z Zhang Chen C Giesy John P JP

Scientific reports 20161116

Solution Phosphorus-31 nuclear magnetic resonance (31P NMR) spectroscopy coupled with enzymatic hydrolysis (EH) with commercially available phosphatases was used to characterize phosphorus (P) compounds in extracts of the dominant aquatic macrophytes and algae in a eutrophic lake. Total extractable organic P (Po) concentrations ranged from 504 to 1643 mg kg-1 and 2318 to 8395 mg kg-1 for aquatic macrophytes and algae, respectively. Using 31P ...[more]

PMID: 27849040

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Dataset Information

Forms and Lability of Phosphorus in Algae and Aquatic Macrophytes Characterized by Solution 31P NMR Coupled with Enzymatic Hydrolysis.

Publications

Forms and Lability of Phosphorus in Algae and Aquatic Macrophytes Characterized by Solution <sup>31</sup>P NMR Coupled with Enzymatic Hydrolysis.

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